Super Resolution via Optical Re-assignment

  • XY resolution exceeding diffraction limit
  • 超分辨率活细胞成像的理想选择
  • 易于使用CSU
  • Upgradable from CSU-W1

XY resolution of approx. 120nm*1

XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology.
Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution.

NG108 cell
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

NG108 cell growth cone

超分辨率活细胞成像的理想选择

就像CSU一样,高速实时成像可以通过超分辨率执行。
In addition, live cell imaging is possible, reducing bleaching and phototoxicity.

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线粒体的实时活细胞成像(10FPS)
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

线粒体的实时活细胞成像(10FPS)

CSU易于使用

Super-resolution images can be observed in real time without any specific preparation of sample.
通过基于共聚焦技术的光学切片使深度观察成为可能。

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3D image

*1 For reference

Details

SoRa super-resolution principle

SoRa super-resolution principle

常规共聚焦显微镜中的图像形成显示为照明PSF(点扩散函数)和检测PSF的乘积。如果我们考虑来自光轴的位置d的针孔上的图像形成,则是照明PSF和检测PSF的乘积(如图所示),我们可以从光轴处看到该信息在d/2的位置在光源处传输。也就是说,在光源处的d/2位置的信息将放大到针孔上的d。为了纠正这一点,安装了微丝片,并将投影到针孔上的单个焦点光学地减小了一半,从而形成了理想的图像形成。
通过这样做,该决议是由大约equal to an ideal confocal microscope, in which the pinhole has been reduced to an infinitesimal size, producing an estimated 1.4x improvement upon regular confocal microscopes.

Reference
T. Azuma and T. Kei, “Super-resolution spinning-disk confocal microscopy using optical photon reassignment,"
Opt. Express 23, 15003-15011 (2015)

Configuration when upgrading from the CSU-W1

Configuration when upgrading from the CSU-W1

A SoRa disk can be added to your CSU-W1.
通过为Sora使用放大倍率的改变器,可以通过在常规共聚焦观察和超分辨率观察之间切换来执行根据实验要求进行量身定制的成像。

1x: Confocal observation (CSU-W1)
2.8x: 超分辨率100X物镜镜头
4x: Super-resolution 60x objective lens

概述 :Confocal scanner unit CSU-W1

连续ct specification

连续ct specification*1
Model 1camera model (T1) 2camera model (T2) 拆分视图模型(T3)
Loadable model A SoRa disk can be loaded as disk 2, and disk 1 can be selected (50μm or 25μm)
激发波长 405nm~640nm
观察波长 420nm~680nm
Effective field of view Depends on the magnification changer for SoRa specification (see below)
外部光线 / NIR端口 An external light port cannot be equipped at the same time as the intermediate magnification switcher
The NIR port cannot be used together with a SoRa disk
Magnification changer for SoRa specification
Lens-switched light path 3 light paths switched electronically  1x, 2.8x, 4.0x magnification
外部尺寸 425(W)×301.1(L)×122.5(H) mm (excl. protrusions and supporting column)
重量 13kg
Microscope connection Manufacturer-specific adapter
使用放大倍率的Sora时的视野
Magnification changer for SoRa 2.8x 4.0x
Recommended objective lens 100x 60x
Effective field of view 61x57μm 71x67μm
Resolution: : PSF FWHM*2
XY/Z分辨率(光学超分辨率) 150nm / 320nm
xy/z分辨率(反卷积后) 120nm / 300nm

*1仅显示与CSU-W1不同的项目。规格 :Confocal scanner unit CSU-W1
*2 Resolution value is for reference only.

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