Single Cellome Unit SU10 enables the delivery of substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells.
For use with an inverted optical microscope. Microscope is not included with the SU10.

SU10的优势

- 单细胞靶向直接递送到核或细胞质中。
- Minimal damage to cells.
- Automated, high speed, and high success rate.

Details

Automated Nano-point Delivery for single cell : SU10

Single-cell targeting with direct delivery into nucleus or cytoplasm

  • Select cells for delivery while observing under a microscope and deliver substances to into nucleus or cytoplasm of targeted cells.
  • Easy Control of XY-position and automatic control of delivery Z-position in software.

SU10直接交付

FITC-labelled dextran solution (molecular weight 70,000) was delivered into HeLa cells.

Minimal damage to cells

  • 纳米木板是一种玻璃移液器,其最小尖端外径为几十纳米,最大程度地减少对细胞的损害。
  • Delivery with high cell viability enables live single-cell analysis.

SU10纳米副词的尖端

The tip of the nanopipette under an electron microscope.

SU10伤害较少

Automated, high speed, and high success rate

  • The SU10 uses automated cell surface detection, insertion, and delivery to the cell. The process takes approximately 10 seconds with a 90% success rate. (Experiment by Yokogawa)
  • 操作s that were previously performed manually by experienced researchers are much easier with SU10.

SU10 nano delivery process

可以针对目标的细胞的示例

  • Cell line (HeLa, HEK293, CHO, MDCK, HepG2, etc.)
  • 原代细胞培养(神经元,肝细胞等)
  • 干细胞系(IPS细胞,ES细胞等)
  • Immune cell lines (T cell, Ba/F3 cell, etc.)
  • Plant cell with cell wall

Examples of materials that can be injected

  • CRISPR-Cas9 (Cas9 RNP)
  • Protein (antibody, GFP, etc.)
  • Nucleic acid (single-stranded DNA, etc.)
  • Fluorescent reagent

应用example

SU10 application

Q&A

Q1。与显微注射有什么区别?

A1.The SU10 lowers the damage to a cell with the nanopipette because its tip size is less than 1/10 of a tip used for microinjection.
Automatic detection of cell surface enables a high success rate of insertion to the intended depth of a cell.
The delivery operation uses an electrical method rather than pneumatic or hydraulic pressure.

Q2. What t is the difference from traditional transfection methods (e.g. transfection reagents, electroporation)?

A2.The SU10 can deliver materials into the selected cells.
The SU10 enables a direct delivery of reagents into cytoplasm or nucleus.

Q3. What is the difference from electroporation?

A3.In addition to the above-mentioned “difference from traditional transfection methods”, due to automated cell surface detection, the suspension of cells is not required during injection.

Q4. What is the max volume of deliver into the cell?

A4.It is estimated to be tens of femtoliter (fL) per second (1fL=1x10-15L).
The volume can be changed by software settings.
*输送量可能因溶质和车辆而异。

Q5. Is the nanopipette disposable?

A5.Yes, but one nanopipette can deliver to 50 cells or more*.
* Experiment using HeLa cells by Yokogawa.

规格

执行器模块 粗略运动(运动执行器) 中风:大约50mm/轴(设置分辨率XYZ轴:0.625μm)
Fine movement (Piezo actuator) 中风:100μm/轴
(setting resolution XYZ axis: 10nm, at penetration and extraction: 1nm)
测量模块 Voltage generation range -10V to +10V (setting resolution: 10mV)
Current measurement range -900NA至 +900NA(设置电流范围:±9V)
电源供应 Power consumption (Main controller + Piezo controller) 100VA or lower
Supply voltage (Main controller) 100 to 200V / 220 to 240VAC (Switching not required)
Supply voltage (Piezo controller) 100至120V / 220至240VAC(下订单时必须指定型号)
电源频率(主控制器 +压电控制器) 50/60Hz
外部尺寸和体重 Main controller 260(W) x 99(H) x 280(D) mm, Approx. 2.8kg
Piezo controller 236(W) x 88(H) x 273(D) mm, Approx. 4.6kg
Actuator module 270*(w)x 219(h)x 245*(d)mm,大约。2.2kg
* In case the X and Y axes move in the direction of the maximum size
Measurement module 85(W) x 30(H) x 43(D) mm, Approx. 0.1kg
Joystick 100(W) x 145(H) x 144(D) mm, Approx. 0.3kg
Safety guard 130(w)x 230(h)x 287(d)毫米,约0.7公斤
纳米夹的尖端外径(在SU10ACC-NP01的情况下) 低于100nm(参考值)
操作Environment 15至35℃,20至70%rh无凝结,高度至2000m
Microscope compatibility For use with an inverted optical microscope. * Microscope is not include with the SU10.
请联系横川,以便将SU10安装在不同的倒立显微镜上。
Installation examples; Olympus IX71, Olympus IX83, Nikon Ti2

Resources

Overview:

该申请说明将介绍SU10的功能,并提供示例,证明使用该技术传递基因组编辑工具(CAS9 RNP)。

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