Single Cellome Unit SU10 enables the delivery of substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells.
For use with an inverted optical microscope. Microscope is not included with the SU10.
- Minimal damage to cells.
- Automated, high speed, and high success rate.
Automated Nano-point Delivery for single cell : SU10
Single-cell targeting with direct delivery into nucleus or cytoplasm
- Select cells for delivery while observing under a microscope and deliver substances to into nucleus or cytoplasm of targeted cells.
- Easy Control of XY-position and automatic control of delivery Z-position in software.
FITC-labelled dextran solution (molecular weight 70,000) was delivered into HeLa cells.
Minimal damage to cells
- Delivery with high cell viability enables live single-cell analysis.
The tip of the nanopipette under an electron microscope.
Automated, high speed, and high success rate
- The SU10 uses automated cell surface detection, insertion, and delivery to the cell. The process takes approximately 10 seconds with a 90% success rate. (Experiment by Yokogawa)
- 操作s that were previously performed manually by experienced researchers are much easier with SU10.
- Cell line (HeLa, HEK293, CHO, MDCK, HepG2, etc.)
- Immune cell lines (T cell, Ba/F3 cell, etc.)
- Plant cell with cell wall
Examples of materials that can be injected
- CRISPR-Cas9 (Cas9 RNP)
- Protein (antibody, GFP, etc.)
- Nucleic acid (single-stranded DNA, etc.)
- Fluorescent reagent
A1.The SU10 lowers the damage to a cell with the nanopipette because its tip size is less than 1/10 of a tip used for microinjection.
Automatic detection of cell surface enables a high success rate of insertion to the intended depth of a cell.
The delivery operation uses an electrical method rather than pneumatic or hydraulic pressure.
Q2. What t is the difference from traditional transfection methods (e.g. transfection reagents, electroporation)?
A2.The SU10 can deliver materials into the selected cells.
The SU10 enables a direct delivery of reagents into cytoplasm or nucleus.
Q3. What is the difference from electroporation?
A3.In addition to the above-mentioned “difference from traditional transfection methods”, due to automated cell surface detection, the suspension of cells is not required during injection.
Q4. What is the max volume of deliver into the cell?
A4.It is estimated to be tens of femtoliter (fL) per second (1fL=1x10-15L).
The volume can be changed by software settings.
Q5. Is the nanopipette disposable?
A5.Yes, but one nanopipette can deliver to 50 cells or more*.
* Experiment using HeLa cells by Yokogawa.
|Fine movement (Piezo actuator)||中风：100μm/轴
(setting resolution XYZ axis: 10nm, at penetration and extraction: 1nm)
|测量模块||Voltage generation range||-10V to +10V (setting resolution: 10mV)|
|Current measurement range||-900NA至 +900NA（设置电流范围：±9V）|
|电源供应||Power consumption (Main controller + Piezo controller)||100VA or lower|
|Supply voltage (Main controller)||100 to 200V / 220 to 240VAC (Switching not required)|
|Supply voltage (Piezo controller)||100至120V / 220至240VAC（下订单时必须指定型号）|
|外部尺寸和体重||Main controller||260(W) x 99(H) x 280(D) mm, Approx. 2.8kg|
|Piezo controller||236(W) x 88(H) x 273(D) mm, Approx. 4.6kg|
|Actuator module||270*（w）x 219（h）x 245*（d）mm，大约。2.2kg
* In case the X and Y axes move in the direction of the maximum size
|Measurement module||85(W) x 30(H) x 43(D) mm, Approx. 0.1kg|
|Joystick||100(W) x 145(H) x 144(D) mm, Approx. 0.3kg|
|Safety guard||130（w）x 230（h）x 287（d）毫米，约0.7公斤|
|Microscope compatibility||For use with an inverted optical microscope. * Microscope is not include with the SU10.
Installation examples; Olympus IX71, Olympus IX83, Nikon Ti2
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
- 一种单细胞分析解决方案，通过自动化特定细胞和细胞内成分的收集来改变药物发现研究的效率 -
- For the creation of a smart cell industry
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